Yeah, you can use it just fine - though I wouldn't put all 32GB in there if the motherboard only supports 16. It wouldn't hurt anything, but the computer probably wouldn't boot. (Or does the motherboard support more and you just have 16? Not sure on that point.)
When you do plug two sticks in, check your manual and see where to plug them in for dual-channel use; it'll probably be A1 and B1, but it could be A1 and A2.
I have four 8gb sticks of ram(32GB of ram, 4x8gb), the mobo says it supports 16gb, but does not specify a maximum per slot. I have put 2 of the sticks into the mobo, one in slot A1, one in slot B1.