if i buy motherboard that doesn't have any sata 6Gb port, how do this affect the gaming experience?
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Most SSDs can top out a 3Gbps SATA link. An SSD on SATA-II will still be loads better than a platter drive on SATA-II but if you're looking to buy an SSD you might as well future proof your PC a bit. What motherboard were you thinking of buying?
No real difference. An HDD can't max out a 3gb/s port, and an SSD will only do so in sequential read/writes. Even then, it's only load times that are really affected by access speeds.