1) The Drive letters are for labeled for Partitions NOT drives!
2) Drives are listed as Drive 0, 1, 2, 3 and so on.
3) If you ONLY have two drives and you are trying to mirror those two then your OS (C Drive) is on one of those and it WILL be erased
4) If you have 3 or more drives what I would do just to be sure is unplug the drive that has the OS on it from the motherboard so that when you go into the Raid Management it will only see the other two drives. Make the raid 1. Once complete turn OFF the PC, Plug back in your OS drive, and turn back on.
If you are trying to make a Raid using a RAID software program within windows it won't even let you touch the drive that has your OS on it because its in use by the OS.