Well, the basic answer would be yes. But the performance depends on what motherboard you have. If it's LGA 1366, then you're fine. Better even. But if it's LGA 1155, 1150, AM3+ etc. then the ram will stop working in dual channel and be forced to work in single channel mode. That's going to reduce performance and speed. Best to leave it at 2 stcks on slots 1 and 3. Maybe buy 2 4gb sticks if you must upgrade your ram.