The only place you might run into a big bottleneck is if you're running two of those cards in SLI, but your motherboard doesn't support SLI. The card and motherboard will work, but their might be a small percent performance drop (as in 5 and below FPS).
In my old rig, I used a PNY GTX 660 in a PCI Express 2.0 Slot.
Also, unless there is a specific reason you're going with the 1 GB, by the 2 GB GTX 650 or maybe Ti/Ti Boost. 1 GB of VRAM would be a bigger bottleneck than a PCI Express 1.0 Slot.