Upgrading motherboard help

most likely yes, but you can try with your current hard drive first, just upgrade the mobo and plug in your hdd and see if it boots, and if not, you will need to reinstal it. and, if you have an OEM copy of windows, you cant use your lisence again as they are tied to the mobo. if you have retail windows, then you can just reinstall and put your license key in and it will work.