Does it matter which PCI Express slots I use

Hi, I have an Asrock FATAL1TY 990FX Motherboard, an EVGA GTX 770 and an Asus PCE-N15 Wireless Network Card. I know that the board has 2 x16 slots, 1 x4 slot and 2 x1 slots. So does that mean I can put the GPU in either for the x16 slots and the Wireless Network Card in either of the x1 slots? Or can the x4 slot be used for a GPU too?
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    Ideally you should put the gpu in the first PCIE x16 slot and the wifi card on the farthest slot available.
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