The usb file read/write/transfer speed will only be as fast as the usb device you are using itself.
So a usb 2.0 pen drive plugged into a 3.0 port, will only perform as fast as the pen drive itself id capable of performing at, at the 2.0 speed. Also remember, all devices have different quality read/write speeds;
If i used my usb 3.0 pen drive in a 3.0 port, i'd get Read-73MB/s - Write-13MB/s
But plugged in a 2.0 port, it is Read-30MB/s - Write-9.8MB/s
Kingston DataTraveler R3.0 G2
Also, copying a folder of a hundred smaller files totalling 1GB would take far longer than a single item of 1GB, near half the speed