depending on how you have your cards plugged into the motherboard... the older one plugged in closer to the cpu. your motherboard my run 16x with one card and 8x,4x with two cards plugged in. without knowing what you motherboard is that would be my guess
Some motherboard do allow you to run sli with out the bridge, they just send the signal through the pci-e lanes. But 99% of the time you will need a bridge to enable sli. Turn off your computer and plug the sli bridge in, if there are two slots it doesn't matter witch one you use just make sure you use the corresponding one on the other card.