No, it's a HDD. HDDs can't saturate SATA-II, so there is no difference between running it at SATA-II vs SATA-III that you would ever notice. In theory there is, but this would be for buffered reads (ie, read a file under 64MB then read it again immediately after).
Any loading time in games would be the same between the same HDD setup on a SATA-II port vs SATA-III port.
SATA-II can only slow down SSDs, but even then you're talking about slowing things down to the point its the fastest the SATA-II connection can support which means it's still much faster than a HDD. Since you typically have SSDs being an upgrade from HDDs, the benefit is very real here. In the situation one had two SSDs, one at SATA-III and one at SATA-II, then yes...