No, it will not work. Look at the mobo's spec page.
https://www.asus.com/Motherboards/B85PRO_GAMER/specifications/
The PCIe 2.0 slot only operates at x4, whereas you need at least x8 for SLI. I think they'd have to both be PCIe 3.0 too, but I'm not sure.
Or, more simply, note that Nvidia SLI is not listed under "Multi GPU support".
Come to think of it, I don't know if any non Z-series Intel mobo supports SLI.