Welcome to the TH community, @wovlerine!
It really depends on the drive as you weren't specific if you are talking about a mechanical hard drive (HDD) or a solid-state drive (SSD)?
You wouldn't have any issues with a mechanical hard drive, even if it's SATA III (6 Gb/s) as its capabilities cannot exceed the bandwidth of a SATA II (3 Gb/s) port either way.
However, if your SATA III drive is an SSD, you will definitely be bottlenecking its performance. Solid-state drives are high-bandwidth devices and can reach transfer spped rate over 500 MB/s! This is something a SATA II port (
3 Gb/s = 300 MB/s) cannot provide.
Keep me posted with the details and let me know if you have more questions!
Best of luck!
SuperSoph_WD