Hey there, Prasathn97.
It really depends on the stereo system in the car. You should check its User Manual, to see what's the supported File System, file types and max flash drive capacity. Those things can vary for different stereo systems. E.g. if the flash drive is formatted with the NTFS file system, but the car's audio system supports only FAT32, or exFAT, this could lead to the issue at hand.
Hope that helps. Please let me know how it goes.
Boogieman_WD