It can depend what kind of RAID card you have but in general you should be perfectly fine. One of the benefits of using a dedicated RAID card is that you can forklift the card and the drives and move them to a totally different system if you want, since the RAID data is on the drives and the volume info is on the RAID card. Windows knows nothing about the array. The RAID card simply presents the volume as a single SCSI disk to the OS, and windows gives it a drive letter.
If you've got a "kinda" RAID card (not a true technical term....) that uses the host machine's CPU for parity & RAM for caching, it "should" act the same way but I'd be a little more nervous. Those cards should also have the logical volume info stored locally on themselves though.
Point is, with a true RAID adapter there should be no issue whatsoever moving it around to another slot. The only thing you need to be concerned about is having enough PCIe lanes/bandwidth in the slot you're connecting to in order to support the throughput of your disk array.
ALLLLL that said, yeah, like others said, doing a backup prior is NEVER a bad idea.