It likely is going to be a massive problem. It is not so much the unknown octets in the first 2 positions it is you not know the subnet mask.
In general a network that functions all devices will have the same subnet mask and the corresponding bit position will be the same. The problem with any scanner software is it must send out ARP messages to find the device. The ARP is sent on the broadcast IP for the subnet. If the subnet mask it wrong it will send the ARP on the wrong ip and the machine will never hear it.