yes
backward compatibility = true
Damn. beat me to it... you wiley minx!
However, You are going to need to do one thing: lock down the data transfer rate on the SATA 3.0Gb/s drive. If you mix speeds or try and use a SATA 3.0Gb/s drive on a SATA 1 controller, there can be issues. It's nothing big, just toss on a jumper on to the appropriate pins. Check your manufacturers website it will tell you.
I have run into this issue on multiple occasions. I had a Raptor (which is SATA 150) and a Samsung (SATA 300) on the same controller (SATA 300) and my computer randomly locked up until I locked down the Samsung's transfer rate to SATA 150.
My friend also had an issue with the Samsung on a SATA 150 controller, but once he locked the down the data rate it was perfect.