Yes, there is a difference. Whether it is worth it or not depends on how picky you are.
Oh, and ih8u, moving to a 250GB drive would actually tend to slow down seeks. The way to improve seeks on a normal drive is to short stroke it - buy a 1TB drive or something similar, and only use the first 200GB (create a partition on the first 200GB or so, and leave the rest untouched). That will decrease seek times significantly, while still being cheaper than a velociraptor (though it will not match a velociraptor for speed).