I'm currently running Windows 7 Home Premium 64-bit (Trim enabled). I did defrag my drive a few times (before I learned your not supposed to), even though Windows shows my drive as heavily fragmented. My SATA controller is configured as IDE. I don't have HD Tune Pro, just the free version. I'll download the trial of the Pro version though.
Tried running HD Tune Pro and although the UAC prompt came up, the program wouldn't start. I'm also running it as an administrator. It's listed in memory, but the program window won't come up. Some of my other programs also got really slow all of the sudden. I'm going to try backing up everything and doing a reinstall of Windows.