It may not work, due to your motherboard's older chipset. Microsoft has got some verifcation software that detects windows keycodes not matching with the installation dvd. The oem systems are bad about forcing you to buy their replacement boards to be legal with your microsoft software, which sometimes cost as much as your original system. So you may be better off selling or returning the e5400.
If you want to try a different board, go with micro atx g31. Sis doesn't make any newer chipsets that I know of for .45nm cpus, so the g31 micro atx boards are your best bet if you're not concerned about reinstalling windows. Select one with 4 sata and maybe one ide port; some don't have ide ports.