If you have a motherboard that uses a dual channel memory controller, then two 1GB DIMMs will give you better performance than one 2GB DIMM (assuming the DIMMs have the same performance specs). The reason for this is that the two-channel controller can read or write two DIMMs in parallel, much like a road with two traffic lanes in each direction can handle twice as many cars.
That having been said, because of CPU caches the overall performance of the system is not very dependent on RAM speeds and you probably won't notice any difference at all unless you run benchmarks.