theoretically it should, but I've never really tested it out. with all the sharp pointy bits inside computers though, it isn't too hard to get a small break in the latex, and that would negate part of the reason you were wearing gloves in the first place. I've always just touched the PSU metal casing while the PC was off, but still plugged in. This discharges any built up charge. If you're really worried about static, get an anti static wrist band, and connect it to your PC case while you work on it. They only cost about $3-6 I think, and are more convenient than gloves.