You run a pretty high risk of making a brick out of the MB using any Windows based BIOS updates. The safest way is using the traditional floppy method.
What version are you running and why do you need to update? There is no reason to update if there is no problem.
I had a Rev. 3.3 of that board for a couple of years with an E6600 and never had any problems with it. I am pretty sure Rev. 1 only supports 1066 fsb while Rev. 3.3 supported 1333 fsb and with F14b BIOS had "unofficial" support for 45nm CPUs ......E7xxx, E8xxx, Q8xxx and Q9xxx series. Even on the Rev.3.3 with that unofficial support I would not run any of those chips though as none of them are unlikely to overclock well due to the P965 chipset limitations. Dont get me wrong P965 was great for first generation 65nm Core2Duo but just was not designed for anything newer.