Usb 3.0 is the new specification on the usb connection which gives you more bandwidth for faster data transfer. To enable usb 3.0 speed your port and device must both be usb 3.0. If your external enclosure are all usb 2.0, they will work on 3.0 port but at the 2.0 speed as usb 3.0 is backward compatible to usb 2.0 and 1.0.
In short, if you don't have usb 3.0 enclosure, you can still use them on 3.0 port, but without the fast bandwidth. Please note that mechanical HDD will be the bottleneck due to the speed limit of the spinning HDD platter and platter density, unless the HDDs are in raid. You will also not see the advertised 4.8 gigabit per second or 600 megabyte per second speed because there is protocol overhead.