Technically speaking, everything will 'work' together (apart from the memory, you'll need DDR2), but it is probably not optimised for it. By that I mean, why use an older X48 chipset based LGA 775 motherboard, when there are newer LGA 1156 & 1366 processors available?
If you can, swap that motherboard out for a GIGABYTE GA-X58A-UD3R, and swap that HDD for a SAMSUNG Spinpoint F3 1TB. As for your CPU you'll need a Core i7 900 based processor, the most ideal would be the i7 930, $200 at Micro Center pickup.
If you're reusing that old board though from an older build, you will need to get a dual channel 2x2GB DDR2 memory kit, 3x2GB is triple channel which is not supported by any Core 2 processors, and that DFI LP DK X48-T2RS board only supports DDR2 memory.