From one first time builder to another, yes.
Current graphics all run through the PCI Express 2.0 slot, of which there may be one, or several on any given motherboard.
Generally, the more important question it one of form. Will the graphics block any other slots on the motherboard? Will the entire assembly fit in the case?
Also, in the case of using SLI or Crossfire, does the motherboard have:
A. Two or more slots
B. Using those slots, and assuming one card doesn't block the other's slot, what will they run in? Most commonly, I've found motherboards will run a single at x16, with two at x8, although, some will put pair at x16 and x8, while other's run at x16, x4, for "power consumption", although that quote may be unreliable, so do your research.
This may garner the "well duh, it's all gotta fit in the case!" response, but it's the same as tech support. Gotta point out the obvious first.
Slazarith