Disk Read Error

Hi,
I recently built a computer using a asus p8z77 board, i5 3550 and seagate barracuda hdd. Raedoen 7850 graphics. After installing windows 7, and booting , everything worked fine. Then this morning, when I booted it up, it said the disk read error press ctrl alt del. I need help.
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More about disk read error
  1. Do you have a usb key in the computer? If so remove the key and reboot, if not can you provide any more details about possible changes to the system since it was working?
  2. I took the USB out after install. All I did was set up account and password then shut down. Now it does that error.
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