There were two slots to put my Twin Frozr II R5850 onto my motherboard. I put it on the slot on the bottom, because my hard drive was in the way of the first slot. Will this matter at all? Or should I move my hard drive and put my graphics card in the first slot?
on that board as long as its in one of the blue slots you're fine
There's only one blue PCIE x16 slot. The second slot is x4 but it is a PCIE 2.0 motherboard. There will be a performance difference but it will be small. Here is an article on the subject where they tested an HD5870; http://www.techpowerup.com/reviews/AMD/HD_5870_PCI-Expr...
A slot like the one your card in limited performance by approximately 5%. The HD5870 needs slightly more bandwidth so it may be a lil lower for your card, unless you have it overclocked and then it could be a slightly larger hit.