M5A99X Evo PCIE Slots - Does it matter which I use?

Hey all. On my motherboard I have three PCI-E slots. Does it matter which I plug my GPU into... like, is one going to be faster than enough by any noticeable measure?
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More about m5a99x pcie slots matter
  1. Always use pcie x16_1 slot for optimal performance when using only one card.
  2. Is that the first slot?
  3. The first blue slot of your mobo is 1st one. It is right below the heatsink.
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