I see a lot of motherboards will offer 2 pcie 2.0 slots, and if you use one of them, it will give you a 16x lane, but if you use both of them (hence sli or crossfire), you only get 8x lanes. Does this hamper performance?
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More aboutmulti graphics cards pcie slots
Generally, no. Maybe a little if you install the top of the line cards from AMD or nVidia in XFire or SLI.