You mean PCI-E 2.1 card on a PCI-E 2.0 board/slot? No worries, it should be backward compatible. They are just basically almost the same thing and there is no differences on bandwidth. For your Information PCI-E generations are known to be backward compatible, mostly don't require BIOS update, but some does. PCI-E 2.0 cards can be put on 1.0, 3.0 can be put on 2.1 or 2.0, and sometimes when you put 2.1 to 1.0 or 3.0 to 1.0 you need BIOS updates.