Are PCIE x16 2.1 cards bottlenecked by PCIE x16 1.0 slots?

Are PCIE x16 2.1 cards bottlenecked by PCIE x16 1.0 slots? I was told that PCIE x16 2.1 cards rarely use up the entire 16 lanes. So a PCIE x16 2.1 cards would just use more lanes in the slower PCIE x16 1.0 slots. I'm sure this will come down how good of a card is plugged into the 1.0 slot. Does anyone what 2.1 cards are bottlenecked by 1.0 slots?
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