If 2 similar GPU, Yes.
If not, AMD or Nvidia ?
If Nvidia, No.
If AMD Depends
If the are the same family, HD5870 and HD5850 or HD5830 yes
If they're not, eg. HD6770 and HD6870, then no
If 2 similar GPU, Yes.
If not, AMD or Nvidia ?
If Nvidia, No.
If AMD Depends
If the are the same family, HD5870 and HD5850 or HD5830 yes
If they're not, eg. HD6770 and HD6870, then no