Can I install a single GPU on PCI-e slot #2?

MOBO = ASUS P877-V Premium


The GPU is hugemongus. It takes up three slots.

I have a CPU Cooler =
But it won't fit if I put the GPU in PCI-e Slot 1

What are the consequences if I put the GPU in Slot2?

I'd rather just get a new CPU Cooler than sacrifice performance..but not sure if that is the case.

Thanks in advance for your input.
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More about install single slot
  1. I run my GPU in slot 2. Its not a huge deal. Just moves the GPU down.
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