Cannot delete a large file (566 Mb).

A folder was installed on my C: drive at 4:13 am that I don't want and wish to delete. When I try to remove this a window comes up that tells me I do not have permission to do this transaction. I tried changing registry permissions with no success.

I would appreciate your help.
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More about cannot delete large file
  1. take ownership of the folder then delete. are you sure the files is not lock(in use)?
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Windows 7